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[摘要]：In this study, a novel mixed-mode composite material, SiO2@P(MAA-co-VBC-co-DVB), was prepared via the hyper-cross-linking of its precursor, which was produced via suspension polymerization in the presence of SiO2 particles. Candida rugosa lipase (CRL) was immobilized on the SiO2@P(MAA-co-VBC-co-DVB) particles via hydrophobic and weak cation-exchange interaction. The resulting immobilized CRL showed much better thermal stability and reusability in comparison to free CRL. On the basis of the excellent biocatalyst prepared, a method for high-efficiency enzymatic esterification of phytosterols with different fatty acids to produce the corresponding phytosterol esters was developed. Six phytosterol esters with conversions above 92.1% and controllable fatty acid composition were obtained under the optimized conditions: 80 mu mol/mL phytosterols, 160 mu mol/mL linolenic acid, and 15 mg/mL CRL@HPCS at 300 rpm and 50 degrees C for 7 h in 30 mL of isooctane. The prepared phytosterol esters possessed a low acid value (<= 0.86 mg of KOH/g), peroxide value (<= 3.3 mequiv/kg), and conjugated diene value (<= 1.74 mmol/kg) and high purity (>= 97.8%) and fatty solubility (>= 28.9 g/100 mL). All the characteristics favored the wide application of phytosterol esters with controllable fatty acid composition in different fields of functional food. |
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