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[摘要]:Reaction of vitamin B-12 (B-12) with excess 2-iodoxybenzoic acid and 2-hydroxypyridine leads to selective oxidation of the 5'-hydroxyl group of the ribose tail of B-12 in a 30% isolated yield. The acid derivative was purified in one step by HPLC chromatography and characterized by MALDI-TOF mass spectrometry, H-1 NMR and 2D (HSQC and HMBC) NMR. The new carboxylic acid derivative is perfectly suited to make stable amide-based B-12 bioconjugates. |
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