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[摘要]：With the goal of controlling stem cell fate, for ultimate application in cell delivery, we synthesized a cell delivery vehicle comprised of an injectable hydrogel of hyaluronan (HA) and methyl cellulose (MC) wherein the MC is modified to allow bio-orthogonal coupling chemistry. Specifically, MC was modified using thiol-maleimide and biotin-streptavidin chemistry to covalently conjugate the cell adhesive peptide, glycine-arginine-glycine-aspartic acid-serine (GRGDS), and the oligodendrocyte-differentiating factor, recombinant platelet-derived growth factor A (rPDGF-A). NSPCs differentiated into significantly more oligodendrocytes when cultured in a HAMC hydrogel modified with both PDGF-A and GRGDS compared to controls of unmodified HAMC hydrogel and HAMC modified with only GRGDS. These results demonstrate both the ability to influence NSPC fate with immobilized growth factor and a platform technology for broad applicability in bioactive factor immobilization. |
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