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Direct immobilization of oxyamine-modified proteins from cell lysates

  作者 YI LONG; CHEN YONGXIANG; LIN POCHIAO; SCHROEDER HENDRIK; NIEMEYER CHRISTOF M; WU YAOWEN; GOODY ROGER S; TRIOLA GEMMA; WALDMANN HERBERT  
  选自 期刊  Chemical Communications;  卷期  2012年48-88;  页码  10829-10831  
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[摘要]Oxyamine-modified proteins can be efficiently and selectively immobilized on ketone-coated glass slides at neutral pH in short reaction times by direct treatment and spotting of protein expression lysates without prior purification.

 
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