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[摘要]：Molecular fluorescent probes have revolutionized biochemical and biophysical studies in the last decades, but with regard to lipids there has been a lack of combining the slim shape of saturated acyl chains with fluorescent properties. Our strategy to pentaene and hexaene fatty acids builds upon commercially available 4-(E)-decenal, which is subjected to a Wittig-Horner reaction after chlorination in alpha-position. DBU-mediated beta-elimination of HCl proceeding the olefination establishes a highly conjugated system to which a salt-free Wittig reaction adds a final double bond leading to a good (Z)-selectivity of 83-86%. The double bond geometry can be optionally isomerized with I-2 to furnish the all-(E)-species. The five conjugated alkene moieties result in a longest-wavelength absorption maximum of about 350 nm. A red-shift to 380 nm was realized by addition of another double bond employing a common Wittig-Horner prolongation sequence. Stokes shifts of about 7300 and 7800 cm(-1), respectively, were observed. |
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