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[摘要]：Regulation of tetrapyrrole biosynthesis in higher plants has been attributed to negative feedback control. Two effectors of feedback inhibition have been identified, heme and the FLU protein. Inhibition by heme implicates the Fe-branch via regulation of the initial step of tetrapyrrole synthesis. In the present work a FLU-containing chloroplast membrane complex was identified, that besides FLU comprises the four enzymes catalyzing the final steps of chlorophyll synthesis. The results support the notion that FLU links chlorophyll synthesis and the target of feedback control, glutamyl-tRNA reductase, thereby allowing also the Mg-branch to control the initial step of tetrapyrrole synthesis. Structured summary of protein interactions: FLU, CHL27, PORB, PORC and CHLP physically interact by blue native page (View interaction) FLU physically interacts with CHL27, PORB, PORC, CHLP and GluTR by anti tag co-immunoprecipitation (View interaction) FLU physically interacts with CHL27 by anti bait co-immunoprecipitation (View Interaction: 1, 2) FLU physically interacts with CHL27 by anti tag co-immunoprecipitation (View interaction) FLU physically interacts with CHL27, PORB, PORC and CHLP by anti tag co-immunoprecipitation (View interaction) (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
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