| |
[摘要]：Hemocyanins are blue copper containing respiratory proteins residing in the hemolymph of many molluscs and arthropods. They can have different molecular masses and quaternary structures. Moreover, several molluscan hemocyanins are isolated with one, two or three isoforms occurring as decameric, didecameric, multidecameric or tubule aggregates. We could recently isolate three different hemocyanin isopolypeptides from the hemolymph of the garden snail Helix lucorum (HIH). These three structural subunits were named alpha(D)-HIH, alpha(N)-HIH and beta-HIH. We have cloned and sequenced their cDNA which is the first result ever reported for three isoforms of a molluscan hemocyanin. Whereas the complete gene sequence of alpha(D)-HIH and beta-HIH was obtained, including the 5' and 3' UTR, 180 bp of the 5' end and around 900 bp at the 3' end are missing for the third subunit. The subunits alpha(D)-HIH and beta-HIH comprise a signal sequence of 19 amino acids plus a polypeptide of 3409 and 3414 amino acids, respectively. We could determine 3031 residues of the alpha(N)-HLH subunit. Sequence comparison with other molluscan hemocyanins shows that alpha(D)-HIH is more related to Aplysia californicum hemocyanin than to each of its own isopolypeptides. The structural subunits comprise 8 different functional units (FUs: a, b, c, d, e, f, g, h) and each functional unit possesses a highly conserved copper-A and copper-B site for reversible oxygen binding. Potential N-glycosylation sites are present in all three structural subunits. We confirmed that all three different isoforms are effectively produced and secreted in the hemolymph of H. lucorum by analyzing a tryptic digest of the purified native hemocyanin by MALDI-TOF and LC-FTICR mass spectrometry. (C) 2011 Elsevier B.V. All rights reserved. |
|
