| |
[摘要]：Alkylresorcinols (ARs) are phenolic lipids that are present in high amounts in the bran layer of different cereals Rye samples, cultivar Hazlet, and a white rye genotype, RT202, were analyzed for their antioxidant properties and AR content and composition, based on six fractions of the bran, where 1 was the outermost fraction and 6 was the bran fraction closest to the endosperm Gas chromatography mass spectrometry (GC-MS) analysis demonstrated that the most commonly found AR homologue in Hazlet rye is C19 0 and that the total amount of ARs decreases from the outermost to innermost fractions The antioxidant activity using oxygen radical absorbance capacity (ORAC) for both white rye genotype RT202 and Hazlet brans was determined to decrease from the outermost fraction,(136 OS mu mol TE/g for Hazlet fraction 1 and,186 mu mol;TE/g for white rye genotype RT202 fraction 1) to the innermost fraction (9 84 mu mol TE/g for Hazlet fraction 6 and 78 75 mu mol TE/g for white rye genotype RT202 fraction 2) A positive relationship was seen with GC-MS results Treatment of PC-12 AC cells with Hazlet fraction 1 increased mitochondrial biogenesis as determined using mitochondrial fluorescent dyes 19 the presence of a prooxidant (AAPH), PC-12 AC cells were better protected from free radical attack when treated with Hazlet fraction 1 then with all other bran fractions The results suggest that higher AR content in bran fractions confers antioxidant protection against free radical damage |
|
