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One- and Two-Photon Live Cell Imaging Using a Mutant SNAP-Tag Protein and Its FRET Substrate Pairs

  作者 ZHANG CHONGJING; LI LIN; CHEN GRACE Y J; XU QINGHUA; YAO SHAO Q  
  选自 期刊  Organic Letters;  卷期  2011年13-16;  页码  4160-4163  
  关联知识点  
 

[摘要]A small molecule-assisted protein labeling strategy based on a mutant SNAP-Tag (mSNAP) and its FRET substrate pairs has been developed. Both one- and two-photon fluorescence microscopic experiments were successfully demonstrated in living cells.

 
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