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Cultivar Evaluation and Effect of Fermentation on Antioxidant Capacity and in Vitro Inhibition of alpha-Amylase and alpha-Glucosidase by Highbush Blueberry (Vaccinium corombosum)

  作者 JOHNSON MICHELLE H; LUCIUS ANITA; MEYER TESSA; DE MEJIA ELVIRA GONZALEZ  
  选自 期刊  Journal of Agricultural and Food Chemistry;  卷期  2011年59-16;  页码  8923-8930  
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[摘要]The berry fruits of highbush blueberry (Vaccinium corymbosum) contain bioactive compounds with potential health benefits. The objective was to evaluate blueberries grown in southern Illinois as well as the effect of fermentation, at two different temperatures, on chemical and physical parameters. Fruits from fifteen blueberry cultivars were analyzed. Fruit diameter ranged from 12.8 mm to 18.7 mm, pH from 2.6 to 3.7, reducing sugars from 6.4% to 15.2%, total sugars from 13.9% to 21.6%, total polyphenols from 0.39 to 1.00 mg gallic acid equivalents (GAE)/g blueberry and antioxidant capacity from 5.8 to 10.9 mu M Trolox equivalents (TE)/g. In vitro alpha-amylase and alpha-glucosidase inhibitory capacity relative to the positive control acarbose, a known anti-diabetic drug, showed a range from 91.8 to 103.3% for alpha-amylase and from 103.2% to 190.8% for a-glucosidase. Wines prepared from several of these blueberry cultivars were analyzed throughout fermentation and compared at room temperature and cold temperature fermentation for pH (3.5 to 6.3), degrees Brix (13.6 to 29.7), total polyphenols (375.4 to 657.1 mu g GAE/mL wine), and antioxidant capacity (4.5 to 25.1 mM TE). The wines were also tested for their in vitro capacity to inhibit alpha-amylase and alpha-glucosidase and maintained similar inhibitory action as the berries. Highbush blueberry cultivars and their fermented beverages are good natural sources of antioxidants and starch-degrading enzyme inhibitors important for type 2 diabetes management.

 
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