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[摘要]：Background: Lignin fractions of Lentinus edodes mycelia extract (LEM) have shown anti-HIV and immunopotentiating activity. However, the action point of lignin-carbohydrate complex has not been elucidated. In order to elucidate their action point, DNA microarray analysis was performed, using mouse macrophage-like J774.1 cells. Materials and Methods: RNA was isolated with Qiagen RNeasy Plus Mini kit, hydridized with Gene Chip Mouse Gene 1.0 ST arrays, and scanned with Affymetrix GeneChip Command Console software. Results: One of the seven lignin-carbohydrate fractions isolated from LEM (Fr4) enhanced the expression of dectin-2 (4.2-fold) and toll-like receptor (TLR)-2 (2.5-fold) prominently, but only slightly modified the expression of dectin-1 (0.8-fold), complement receptor 3 (0.9-fold), TLR1, 3, 4, 9 and 13 (0.8- to 1.7-fold), spleen tyrosine kinase (Syk)b, zeta-chain (TCR) associated 70 kDa protein kinase (Zap 70), Janus tyrosine kinase (Jak)2 (1.0- to 1.2-fold), nuclear factor (Nf)kappa b1, NF kappa b2, reticuloendotheliosis viral oncogene homolog (Rel)a, Relb (1.0- to 1.6-fold), Nf kappa bia, Nf kappa bib, Nf kappa bie, Nf kappa bil2 and Nf kappa biz (0.8- to 23-fold). On the other hand, lipopolysaccharide did not affect the expression of dectin-2 or TLR-2. Conclusion: These data suggest the significant role of the activation of the dectin-2 signaling pathway in the action of lignin-carbohydrate complex on macrophages. |
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