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Cantilever-type electrode array-based high-throughput microparticle sorting platform driven by gravitation and negative dielectrophoretic force

  作者 Kim, Y; Lee, J; Kim, Y; Shin, SM; Kim, B  
  选自 期刊  Journal of Micromechanics and Microengineering;  卷期  2011年21-1;  页码  15015-15015  
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[摘要]In this paper, we describe a cantilever-type electrode (CE) array-based high-throughput sorting platform, which is a tool used to separate microparticles using gravitation and negative dielectrophoretic (n-DEP) force. This platform consists of meso-size channels and a CE array, which is designed to separate a large number of target particles by differences in their dielectric material properties (DMP) and the weight of the particles. We employ a two-step separation process, with sedimentation as the first step and n-DEP as the second step. In order to differentiate the weight and the DMP of each particle, we employ the sedimentation phenomena in a vertical channel and the CE-based n-DEP in an inclined channel. By using three kinds of polystyrene beads with diameters of 10, 25 and 50 mu m, the optimal population (107 beads ml(-1)) of particles and the appropriate length (25 mm) of the vertical channel for high performance were determined experimentally. Conclusively, by combining sedimentation and n-DEP schemes, we achieve 74.5, 94.7 and 100% separation efficiency for sorting microparticles with a diameter of 10, 25 and 50 mu m, respectively.

 
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