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[摘要]：In mammals, the duration of the cycle of the seminiferous epithelium (DCSE) largely differs between species, but is remarkably stable within a species, usually showing variations of 1%-3% It is difficult to change the DCSE, e g, by hormones or chemicals Initial experiments, employing quantitative RTPCR, aimed at investigating the diurnal profiles of the clock genes Arntl (previously called Bmal1) and Per1 in testes and kidneys of Djungarian hamsters (Phodopus sungorus) While the testicular levels of Arntl were almost constant, clear diurnal variations were identified for Pen 1 In order to clarify whether day length (T-cycle) is a factor for DSCE, adult male hamsters (n = 20 per group) were exposed to normal (T = 24 h), prolonged (T = 25 h), or shortened (T = 23 h) T-cycles, with cycles thus being longer or shorter by 4 2% compared to the normal condition Exposure lasted for 43 days, during which the activity of the animals was recorded to confirm entrainment DCSE was estimated by incorporation of bromodeoxyuridine in dividing cells and the immunohistochemical localization of labeled cells in stages I-XII of the seminiferous epithelium Despite the low variability of the results and the close agreement with previously published data, no effects of prolonged or shortened T-cycles on DCSE could be identified (24 h 7 98 +/- 0 05 days, 23 h 7 94 +/- 0 04 days, 25 h 7 91 +/- 0 03 days, P > 0 05) The results strongly indicate that the high temporal precision of spermatogenesis is independent of the central circadian clock |
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