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[摘要]：Aim: The purpose of this study was to examine the effects of combined treatment with a specific type of sulfoglycolipid, alpha-sulfoquinovosylmonoacylglycerol (alpha-SQMG), and X-irradiation (XRT) on the remodeling of tumor microenvironments. Materials and Methods: A human colon cancer cell line, SW480, was used in this study. The cells were injected subcutaneously into nude mice and the resulting tumors were treated with alpha-SQMG that was injected intravenously and/or X-irradiation (XRT). The tumor volumes were monitored and the microenvironments of the treated tumors were immunohistochemically analyzed for angiogenesis, pericyte recruitment, and hypoxic fractions using markers for CD31, collagen IV, alpha-Sma, and pimonidazole. Results: The combined treatment with alpha-SQMG (five daily injections from days zero to four) and X-irradiation (two fractions on days zero and three) synergistically enhanced the radioresponse of tumor growth in vivo, whereas alpha-SQMG treatment alone had no effect. The tumor vessel density was significantly decreased at days 10 and 20 after initiating the combined treatment. On day 20, areas with overlapping CD31 and collagen IV expression were rarely observed, suggesting that the normal structures of most tumor vessels had collapsed. alpha-Sma staining was significantly increased and pimonidazole staining was significantly reduced at 24 and 72 11, but not 6 h, after the first combined treatment. Conclusion: The combined treatment induced remodeling of the microenvironments in SW480 tumors, which might contribute to the radiosensitization to the second irradiation. |
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