Characterization of xenobiotic reductase A (XenA): study of active site residues, substrate spectrum and stability

[摘要]：Xenobiotic reductase A (XenA) has broad catalytic activity and reduces various alpha,beta-unsaturated and nitro compounds with moderate to excellent stereoselectivity. Single mutants C25G and C25V are able to reduce nitrobenzene, a non-active substrate for the wild type, to produce aniline. Total turnover is dominated by chemical rather than thermal instability.

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