Highly efficient chemoenzymatic synthesis of beta 1-3-linked galactosides

[摘要]：A novel D-galactosyl-beta 1-3-N-acetyl-D-hexosamine phosphorylase cloned from Bifidobacterium infantis (BiGalHexNAcP) was used with a recombinant E. coli K-12 galactokinase (GalK) for efficient one-pot two-enzyme synthesis of T-antigens, galacto-N-biose (Gal beta 1-3GalNAc), lacto-N-biose (Gal beta 1-3GlcNAc), and their derivatives.

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