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[摘要]：Maitotoxin (MTX), a potent polyether marine biotoxin, induces Ca2+ entry in different mammalian cells by activation of Ca2+ channels. The identity and modulation of the MTX-activated Ca2+ entry pathway is not known. In this work, we show, for the first time, that glucose and lactate can modulate the excitability of spermatogenic cell MTX-activated Ca2+ channels. Physiological and pharmacological evidences indicate that glucose and lactate differentially affect MTX-activated Ca2+ entry mainly through changes that these substrates induce on intracellular Ca2+ stores and the concentration of intracellular Ca2+ ([Ca2+](i)) in spermatogenic cells. Our findings strongly suggest that MTX-activated Ca2+ channels in spermatogenic cells can be regulated by a Ca2+-CaM-dependent protein kinase. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
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