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[摘要]：There is mounting evidence that peroxynitrite (ONOO-) is closely related to the pathogenesis of various diseases. As a pharmacological strategy aimed at preventing ONOO--mediated toxicity, the protective activity of Forsythia suspensa (Thunb.) Vahl (Oleaceae) against ONOO--induced cellular damage was investigated and its active components identified. After bioactivity-guided fractionation of its methylene chloride fraction, two tetrahydrofurofuran lignans were isolated, namely phillygenin and 8-hydroxypinoresinol. The protective effects of these lignans against ONOO--induced cell death were evaluated using renal epithelial cell LLC-PK1. Phillygenin and 8-hydroxypinoresinol significantly reduced the cell injury by 3-morpholinosydnonimine (SIN-1), a ONOO- generator. The hydroxy substituents on the phenyl moieties may contribute to the antioxidant activities of these lignans. These results suggest that phillygenin and 8-hydroxypinoresinol may be useful for the therapeutic or preventive applications in treating ONOO--related diseases. Copyright (C) 2009 John Wiley & Sons, Ltd. |
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