| |
[摘要]：Suppression of activation or fibrogenesis and induction of apoptosis, in hepatic stellate cells (HSCs) have been proposed as therapeutic strategies against liver fibrosis. Curcumin, an active compound isolated from yellow curry pigment of turmeric (Curcuma longa Linn), has been demonstrated to be an effective anti-inflammatory and antioxidant compound. In this study, we investigated the in vitro antifibrogenic effects of curcumin on HSCs at the concentration range of (140 mu M). A cell line of rat HSCs (HSC-T6) was stimulated with transforming growth factor-beta 1 (TGF-beta 1). The inhibitory effects of curcumin (1.25 similar to 10 mu M) on fibrosis-related markers including alpha-smooth muscle actin (alpha-SMA) and collagen were assessed. In addition, the induction effects of curcumin (20 similar to 40 mu M) on apoptosis in HSC-T6 cells were also assessed by Hoechst and propidium iodide stains. Curcumin (1.25 similar to 10 mu M) concentration-dependently suppressed TGF-beta 1-induced alpha-SMA expression and collagen deposition in HSC-T6 cells, without cytotoxicity. Whereas, higher concentrations of curcumin (20 similar to 40 mu M) induced cell apoptosis and cytochrome c release in HSC-T6 cells. Our results suggest that curcumin exerted antifibrotic effects, possibly through two different mechanisms depending on its concentrations. At lower concentrations (1.25 similar to 10 mu M), curcumin exerted antifibrogenic effects, whereas at higher concentrations (20 similar to 40 mu M), curcumin exerted induction of apoptosis in HSCs. Copyright (C) 2009 John Wiley & Sons, Ltd. |
|
