Kinetic resolution of aliphatic acyclic beta-hydroxyketones by recombinant whole-cell Baeyer-Villiger monooxygenases-Formation of enantiocomplementary regioisomeric esters

[摘要]：A set of various linear aliphatic beta-hydroxyketones was investigated as substrates in the enzymatic kinetic and regioselective Baeyer-Villiger oxidation catalyzed by 12 Baeyer-Villiger monooxygenases from different bacterial origin. Excellent enantioselectivities (E > 100) could be observed with 4-hydroxy-2-ketones. After acyl migration, the ester undergoes hydrolysis followed by the formation of optically active 1,2-diols. Furthermore, resolution of 5-hydroxy-3-ketones gave access to the 'abnormal' esters, which broadens applicability of these enzymes in organic chemistry. Additionally, it was noticed, that several substrates were converted by different enzymes in an enantiocomplementary way and with high optical purities. (C) 2009 Elsevier Ltd. All rights reserved.

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