[摘要]：Low temperature and noxious chemicals activate transient receptor potential (TRP) A1 channel in spinal nociceptive neurons. TRPA1 ligands are found as common ingredients of pungent spices and can trigger autonomic reflexes and alter blood pressure. Recent discovery of TRPA1 on visceral afferent pathway suggests such nonnociceptive responses may be closely related to TRPA1. To understand potential contributions of TRPA1 on autonomic response, we have tested TRPA1 agonist allyl isothiocyanate (AITC) effects on mechanically dispersed nucleus tractus solitarii neurons. AITC of 200 mu M increased glutamate release in 58% of tested neurons and TRP channel antagonist ruthenium red (50 mu M) blocked the AITC actions. tetrodotoxin and Cd2+ did not eliminate AITC-evoked glutamate release increase. TRPA1 expressed in capsaicin-sensitive and insensitive neurons. NeuroReport 20:1002-1006 (C) 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins.
