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[摘要]：The importance of studying chromosome structure is first described. Then an overview of historical imaging methods that enable us to quantitatively understand chromosome images and structure is given with special reference to the identification of small plant chromosomes and development of their quantitative chromosome maps. A three-dimensional understanding of chromosome distribution within a nucleus answers why the gene-rich regions localize at both ends of chromosomes, especially in the case of species with Rabl orientation. Not only imaging methods but also proteomic approaches are effective in understanding chromosome structure. Over 200 proteins have been identified by proteome analysis of human metaphase chromosomes, and are categorized into 4 distinct groups according to their nature and localization on chromosomes. These are chromosome coating proteins (CCPs), chromosome peripheral proteins (CPPs), chromosome structural proteins (CSPs), and chromosome fibrous proteins (CFPs). A chromosome 4 layer model has been developed accordingly. Case studies on individual identified proteins are further described, and the functional similarities of CPPs are exemplified. In addition the controversial roles of CSPs (topoisomerase and condensin), especially for development of higher-order chromosome structure, are discussed. Finally, it is concluded that further advances in chromosome research are necessary to solve an enigma lasting nearly 2 centuries, that is, why chromosomes retain the same shape in plants and animals. Copyright (C) 2009 S. Karger AG, Basel |
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