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[摘要]：The authors have demonstrated the generation of a novel hydrogel by the simple expedient of mixing two cationic polymers, chitosan and poly(ethylenimine), in the presence of cell culture media to give a 3D scaffold. The hydrogels were stable under cell culture conditions and were fully capable of supporting cell attachment and proliferation. This gel represents a new class of materials prepd. in a simple, robust, and scalable manner and offers the ability of manipulating the cells in their 3D state, with the ability to generate gels of any desired shape by using a mold. A variety of addnl. materials such as DNA, proteins, peptides, and small drug mols. can be stably incorporated into the gel network offering the potential of modulating cellular growth/behavior within the gel. Culture of primary skeletal cells within the 3D gel environment facilitated cell proliferation and prevented dedifferentiation of the skeletal cells into fibroblasts by maintaining these cells in a chondrocyte-like spherical morphol. |
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