Three-dimensional ultralarge-pore Ia3d mesoporous silica with various pore diameters and their application in biomolecule immobilization.

[摘要]：Highly ordered mesoporous three-dimensional Ia3d silica (KIT-6) with different pore diams. has been synthesized by using pluronic P123 as surfactant template and n-butanol as cosolvent at different synthesis temps. in a highly acidic medium. The materials were characterized by XRD and N2 adsorption. The synthesis temp. plays a significant role in controlling the pore diam., surface area, and pore vol. of the materials. The material prepd. at 150癈, KIT-6-150, has a large pore diam. (11.3 nm) and a high specific pore vol. (1.53 cm3g-1). We also demonstrate immobilization of lysozyme, which is a stable and hard protein, on KIT-6 materials with different pore diams. The amt. of lysozyme adsorbed on large-pore KIT-6 is extremely large (57.2 mmolg-1) and is much higher than that obsd. for mesoporous silicas MCM-41, SBA-15, and KIT-5, mesoporous carbons, and carbon nanocages. The effect of various parameters such as buffer concn., adsorption temp., concn. of the lysozyme, and the textural parameter of the adsorbent on the lysozyme adsorption capacity of KIT-6 was studied. The amt. adsorbed mainly depends on soln. pH, ionic strength, adsorption temp., and pore vol. and pore diam. of the adsorbent. The mechanism of adsorption on KIT-6 under different adsorption conditions is discussed. In addn., the structural stability of lysozyme mols. and the KIT-6 adsorbent before and after adsorption were investigated by XRD, nitrogen adsorption, and FTIR spectroscopy.

[全文传递流程]：

一般上传文献全文的时限在1个工作日内