【文章名】Structure-Activity Relationship Studies for the Peptide Portion of the Bladder Epithelial Cell Antiproliferative Factor from Interstitial Cystitis Patients.
Structure-Activity Relationship Studies for the Peptide Portion of the Bladder Epithelial Cell Antiproliferative Factor from Interstitial Cystitis Patients.
作者
Kaczmarek, Piotr;Keay, Susan K.;Tocci, Gillian M.;Koch, Kristopher R.;Zhang, Chen-Ou;Barchi, Joseph J., Jr.;Grkovic, David;Guo, Li;Michejda, Christopher J.;
[摘要]：We performed comprehensive structure-activity relationship (SAR) studies on the peptide portion of antiproliferative factor (APF), a sialylated frizzled-8 related glycopeptide that inhibits normal bladder epithelial and urothelial carcinoma cell proliferation. Glycopeptide derivs. were synthesized by solid-phase methods using std. Fmoc chem. and purified by RP-HPLC; all intermediate and final products were verified by HPLC-MS and NMR analyses. Antiproliferative activity of each deriv. was detd. by inhibition of 3H-thymidine incorporation in primary normal human bladder epithelial cells. Structural components of the peptide segment of APF that proved to be important for biol. activity included the presence of at least eight of the nine N-terminal amino acids, a neg. charge in the C-terminal amino acid, a free amino group at the N-terminus, maintenance of a specific amino acid sequence in the C-terminal tail, and trans conformation for the peptide bonds. These data provide crit. guidelines for optimization of structure in design of APF analogs as potential therapeutic agents.
