【文章名】Biotin Sulfone as a New Tool for Synthetic Oligosaccharide Immobilization: Application to Multiple Analysis Profiling and Surface Plasmonic Analysis of Anti-Candida albicans Antibody Reactivity against a and b (1?) Oligomannosides.
Biotin Sulfone as a New Tool for Synthetic Oligosaccharide Immobilization: Application to Multiple Analysis Profiling and Surface Plasmonic Analysis of Anti-Candida albicans Antibody Reactivity against a and b (1?) Oligomannosides.
作者
Collot, Mayeul;Sendid, Boualem;Fievez, Aurelie;Savaux, Camille;Standaert-Vitse, Annie;Tabouret, Marc;Drucbert, Anne Sophie;Danze, Pierre Marie;Poulain, Daniel;Mallet, Jean-Maurice;
[摘要]：and basic anal. of yeast-related pathogenic mechanisms, a library of 1? oligomannosides suitable for immunoanal. was prepd. The use of biotin sulfone, an oxidized form of biotin, offers a convenient soln. for both oligosaccharide synthesis and immobilization on microspheres and surface plasmon resonance sensors. The application of this new strategy for the anal. of anti-Candida albicans antibody response through multiple-analyte profiling technol. (Luminex) and with surface plasmonic anal. using biotin tagged synthetic oligosaccharides on avidin coated surfaces was validated using monoclonal antibodies.
