Highly sensitive and fast responsive fluorescence turn-on chemodosimeter for Cu2+ and its application in live cell imaging.

[摘要]：A rhodamine B deriv. I contg. a highly electron-rich S atom has been synthesized as a fluorescence turn-on chemodosimeter for Cu2+. Following Cu2+-promoted ring-opening, redox and hydrolysis reactions, comparable amplifications of absorption and fluorescence signals were obsd. upon addn. of Cu2+; this suggests that chemodosimeter I effectively avoided the fluorescence quenching caused by the paramagnetic nature of Cu2+. Importantly, I can selectively recognize Cu2+ in aq. media in the presence of other trace metal ions in organisms (such as Fe3+, Fe2+, Cu+, Zn2+, Cr3+, Mn2+, Co2+, and Ni2+), abundant cellular cations (such as Na+, K+, Mg2+, and Ca2+), and the with high sensitivity (detection limit ?0 ppb) and a rapid response time (? min). Moreover, by virtue of the chemodosimeter as fluorescent probe for Cu2+, confocal and two-photon microscopy expts. revealed a significant increase of intracellular Cu2+ concn. and the subcellular distribution of Cu2+, which was internalized into the living HeLa cells upon incubation in growth medium supplemented with 50 mM CuCl2 for 20 h.

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