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[摘要]：Incubations of linoleic acid with cell-free preparations from Lily-of-the-Valley (Convallaria majalis L, Ruscaceae) roots revealed the presence of 13-lipoxygenase and divinyl ether synthase (DES) activities. Exogenous linoleic acid was metabolized predominantly into (9Z,11E,1'E)-12-(1'-hexenyloxy)-9,11-dodecadienoic (etheroleic) acid. Its identification was confirmed by the data of ultraviolet spectroscopy, mass spectra, H-1 NMR, COSY, catalytic hydrogenation. The isomeric divinyl ether (8E,1'E,3'Z)-1 2-(1',3'-nonadienyloxy)-8-nonenoic (colneleic) acid was detected as a minor product. Incubations with linoleic acid hydroperoxides revealed that 13-hydroperoxide was a preferential substrate, while the 9-hydroperoxide was utilized with lesser efficiency. (C) 2008 Elsevier Ltd. All rights reserved. |
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