[摘要]：We investigated the effect of amido-(NP-OH) and carbamate linkers (NP-HAPC) in nanoparticles composed of hydroxyethylated cationic cholesterol on siRNA transfection. The presence of NaCl in forming a NP-OH nanoplex increased the suppressive effect of gene expression by increasing the size of the nanoplex and changing the cellular uptake mechanism from membrane fusion and clathrin-mediated endocytosis to clathrin-and caveolae-mediated endocytosis. (C) 2008 Elsevier Ltd. All rights reserved.
