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[摘要]：Adaptor protein-1 (AP-1) is recruited onto the trans-Golgi network via binding to Arf-1GTP cargo-sorting signals and phosphoinositides, where it orchestrates the assembly of clathrin-coated vesicular carriers that transport cargo molecules to endosomes. Here we show that cytosolic AP-1 polymerizes when recruited onto enriched Golgi membranes and liposomes containing covalently attached cargo-sorting signal peptides. Incubation of cytosolic or purified AP-1 With Soluble sorting signal peptides also resulted in AP-1 polymerization, showing that Arf-1 GTP and membranes are not required for this process. We propose that cargo-induced polymerization of AP-1 contributes to stabilization of the coat complex in the formation of clathrin-coated buds. (c) 2008 Elsevier Inc. All rights reserved. |
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