Torque-Induced Slip of the Rotary Motor F-1-ATPase

[摘要]：F-1-ATPase plays an essential role in cellular metabolism by linking rotational motion to ATP hydrolysis/synthesis We measure the torque profile of F, in both ATP hydrolysis and synthesis directions using a novel magnetic nanorod assay F, is found to decouple ATP synthesis from rotary motion at a surprisingly low torque This low-torque slip mechanism protects the enzyme from excessive load and may play a broader biological role by reducing the production of reactive oxygen species

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