[摘要]：L-Xylulose reductase is part of the eukaryotic pathway for L-arabinose catabolism. A previously identified L-xylulose reductase in Hypocrea jecorina turned out to be not the 'true' one since it was not upregulated during growth on L-arabinose and the deletion strain showed no reduced L-xylulose reductase activity but instead lost the D-mannitol dehydrogenase activity [17]. In this communication we identified the 'true' L-xylulose reductase in Aspergillus niger. The gene, lxrA (JGI177736), is upregulated on L-arabinose and the deletion results in a strain lacking the NADPH-specific L-xylulose reductase activity and having reduced growth on L-arabinose. The purified enzyme had a K-m for L-xylulose of 25 mM and a nu(max) of 650 U/mg. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
