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[摘要]：The human fatty acid binding protein (FABP2) is involved in intestinal absorption and intracellular trafficking of long-chain fatty acids. Here we investigate transcriptional regulation of FABP2 by the endodermal hepatic nuclear factor 1 alpha (HNF-1 alpha). In electromobility shift and supershift assays we show the presence of two adjacent HNF-1 alpha binding sites within the FABP2 promoter regions -185 to -165 and -169 to -149. HNF-1 alpha activates an FABP2 promoter luciferase construct by 3.5 and 20-fold in Caco-2 and Hela cells, respectively. Mutational analysis of HNF-1 alpha elements resulted in about 50% reduction of basal and HNF-1 alpha induced activity of FABP2 promoter constructs, predominantly caused by deletion of the -185 to -165 site. Thus, our data suggest a major role of HNF-1 alpha in control of FABP2 expression in intestine via a functional HNF-1 alpha recognition element within FABP2 promoter region -185 to -165. (C) 2008 Elsevier B.V. All rights reserved. |
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