[摘要]:Bioassay-guided separation by use of the fission yeast expressing NES of Rev, an HIV-1 viral regulatory protein, disclosed 1'-acetoxychavicol acetate (ACA, 1) as a new inhibitor for nuclear export of Rev from the roots of Alpinia galanga. Both analysis for mechanism of action with biotinylated probe ( 2) and several synthesized analogs established crucial portions in 1 for Rev-export inhibitory activity. (C) 2009 Elsevier Ltd. All rights reserved.