[摘要]:Direct CD4(+) T lymphocytes were separated from whole mouse splenocytes using 1-dimensional ferromagnetic nickel silicide nanowires (NiSi NWs). NiSi NWs were prepared by silver-assisted wet chemical etching of silicon and subsequent deposition and annealing of Ni. This method exhibits a separation efficiency of similar to 93.5%, which is comparable to that of the state-of-the-art superparamagnetic bead-based cell capture (similar to 96.8%). Furthermore, this research shows potential for separation of other lymphocytes, B, natural killer and natural killer T cells, and even rare tumor cells simply by changing the biotin-conjugated antibodies. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4704924]