[摘要]：This study was undertaken to evaluate the role of collagen matrix to enhance platelet-rich plasma (PRP) effects on pro-inflammatory cytokine-induced arthritic model. We have previously demonstrated the highly regenerative roles of PRP to restore disc degeneration and osteoporosis. In this study. PRP modulated by collagen matrix was used as a regenerative and anti-inflammatory mediator to rescue the chondrocyte degeneration induced by pro-inflammatory cytokines IL-1 beta (10 ng/ml)+TNF-alpha (20 ng/ml). First, the mu result indicated that 1 ng/ml TGF-beta 1 in PRP showed an optimal dosage for chondrocytes proliferation. The chondrogenic-specific gene expressions were rescued by PRP from the inhibition of IL-1 beta+TNF-alpha, especially under the modulation of collagen matrix. The inflammatory molecules activated by IL-1 beta+TNF-alpha were also significantly diminished by PRP with collagen matrix. The membrane receptors integrin alpha 1 beta 1 and CD44 were strongly inhibited by IL-1 beta+TNF-alpha, while this inhibition was then recovered by PRP in collagen coating condition. In a 3D model encapsulated with collagen, PRP-induced chondrogenesis were highly enhanced, such as strong restoration of type II collagen and proteoglycan from the inhibition of IL-1 beta+TNF-alpha. The result indicated that collagen matrix enhances the effect of PRP on chondrogenesis in response to pro-inflammatory cytokines. The combination of PRP and collagen matrix might facilitate a physiological microenvironment beneficial for maintaining chondrocyte homeostasis and represents an advanced osteoarthritis therapy for clinical applications. (C) 2011 Elsevier Ltd. All rights reserved.
