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作者 |
Brenker, C; Goodwin, N; Weyand, I; Kashikar, ND; Naruse, M; Krahling, M; Muller, A; Kaupp, UB; Strunker, T |
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[摘要]：The sperm-specific CatSper channel controls the intracellular Ca2+ concentration ([Ca2+](i)) and, thereby, the swimming behaviour of sperm. In humans, CatSper is directly activated by progesterone and prostaglandins-female factors that stimulate Ca2+ influx. Other factors including neurotransmitters, chemokines, and odorants also affect sperm function by changing [Ca2+]i. Several ligands, notably odorants, have been proposed to control Ca2+ entry and motility via G protein-coupled receptors (GPCRs) and cAMP-signalling pathways. Here, we show that odorants directly activate CatSper without involving GPCRs and cAMP. Moreover, membrane-permeable analogues of cyclic nucleotides that have been frequently used to study cAMP-mediated Ca2+ signalling also activate CatSper directly via an extracellular site. Thus, CatSper or associated protein(s) harbour promiscuous binding sites that can host various ligands. These results contest current concepts of Ca2+ signalling by GPCR and cAMP in mammalian sperm: ligands thought to activate metabotropic pathways, in fact, act via a common ionotropic mechanism. We propose that the CatSper channel complex serves as a polymodal sensor for multiple chemical cues that assist sperm during their voyage across the female genital tract. The EMBO Journal (2012) 31, 1654-1665. doi: 10.1038/emboj.2012.30; Published online 21 February 2012 |
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