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[摘要]：The mycotoxin, patulin (4-hydroxy-4H-furo[3,2c] pyran-2[6H]-one), is produced by a number of fungi common to fruit- and vegetable-based products, most notably, apples. A rapid, simple, and economical method was described for determination of patulin in apple products. The sample was extracted with ethyl acetate and then the extract was cleaned up by extraction with a sodium carbonate solution. Patulin was determined by reverse-phase liquid chromatography using a C18 column and a photodiode array detector and ultra performance liquid chromatography with electrospray-tandem mass spectrometry. Patulin was separated by an HPLC/DAD method on 1254mm I.D Purospher Star RP-18 endcapped column (Merck) with 5m C18 stacionary phase and it was detected at 275nm. The mobile phase used was acetonitrile-water containing 1% acetonitrile (5/95v/v) and the flow-rate was 0.5mL/min. Ultra performance liquid chromatography (UPLC) was performed on an Acquity UPLC BEH C18 (100mm2.1mm) column. The mobile phase was acetonitrile -0,01mol/L amonium acetate (95/5v/v) for UPLC/MS/MS analysis and the flow-rate was 0.3mL/min. The limits of detection for patulin were found to be 1g/kg for the HPLC/DAD method and 10g/kg for the UPLC/MS/MS method. |
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