[摘要]:Flamvelutpenoids AD (14), four new cuparene-type sesquiterpenes, were isolated from the solid culture of Flammulina velutipes. Their structures were elucidated by NMR experiments. The absolute configurations of 1 and 2 were assigned via the circular dichroism data of the [Rh2(OCOCF3)4] complex, whereas that of C(3) of 3 was determined by applying the octant rule for the a,beta-unsaturated ketone moiety. Compounds 14 showed weak antibacterial activity against Escherichia coli, Bacillus subtilis, and methicillin-resistant Staphylococcus aureus with MIC values larger than 100 mu M.