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[摘要]：It has been reported that levels of viremia reflect the severity of illness in dengue virus infection. We assessed the levels of viremia in patients with primary and secondary infections, using 2 cell lines: Fc gamma R-expressing BHK cells and Fc gamma R-negative cells. In primary infection, virus titers were at similar levels between Fc gamma R-expressing and Fc gamma R-negative cells. In secondary infection, however, virus titers were similar to 10 times higher in Fc gamma R-expressing cells on days 1-6 when compared with Fc gamma R-negative cells, indicating discrepancy in viremia titers between Fc gamma R-expressing and Fc gamma R-negative cells. The results suggest that dengue virus-antibody complexes with infectious capacity exist in patients with secondary infection, and these immune complexes can be detected by using Fc gamma R-expressing cells. As it has been reported that principal target cells of dengue virus infection are Fc gamma R-positive, monocyte/macrophage lineage cells, virus titers determined using Fc gamma R-expressing cells may better reflect the actual viremic conditions in vivo. |
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