[摘要]：Background: Radiofrequency ablation (RFA) is one treatment option for hepatocellular carcinoma (HCC) where tumour cells are destroyed by heat. However, there is lack of knowledge about cellular reactions after heating. Therefore, we studied cell death after heat application in a cell-culture setting mimicking HCC. Materials and Methods: Intracellularly stained hepatic stellate cells (LX-1) and HCC cells (HepG2) were cultivated in co-culture or alone. Apoptosis was determined by flow cytometry using AnnexinV-PE and eFluor (R) 450. Results: Heating resulted in early apoptosis for 20-30% of HepG2 cells and 10-15% of LX-1 cells. Late apoptosis was observed in a large percentage of cells 24 h after heating at 65 degrees C for 15 min or 75 degrees C for 5 min; 65 degrees C for 10 min resulted in a moderate increase and 55 degrees C for 15 min resulted in a minor percentage of late apoptotic cells. Conclusion: Heat-treated LX-1 and HepG2 cells die by apoptosis. This,finding is important for future planning tools to ameliorate RFA outcome in clinic.
