Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein

[摘要]：A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.

[全文传递流程]：

一般上传文献全文的时限在1个工作日内