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[摘要]：A novel method based on high performance liquid chromatography with variable wavelength detection was developed for quantitative analysis of the bioactive ingredients in a Chinese herbal preparation called Kangnaoshuai capsule (KNS). Seven bioactive ingredients, namely puerarin, albiflorin, stilbene glucoside, salvianolic acid B, baicalin, baicalein, and wogonin were simultaneously determined. The separation was carried out on a RP-C18 column with acetonitrile and 0.1% phosphoric acid as mobile phase under gradient conditions. Five detection wavelengths, 248nm for puerarin, 230nm for albiflorin, 320nm for stilbene glucoside, 275nm for baicalin, baicalein and wogonin, and 286nm for salvianolic acid B, were utilized during a single elution process. The validation data proved the developed method to be sensitive, precise, accurate, and selective, and was successfully applied for the determination of commercial KNS samples from different production batches and different manufacturers. |
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