[摘要]：Protein kinase C (PKC) delta is a regulator of apoptosis with both pro-and anti-apoptotic effects. The mechanistic basis for the discrepant effects is not completely understood. Here we show that Smac interacts with PKC delta. The interaction depends on the N-terminus of Smac and is disrupted upon treatment with paclitaxel. This is associated with release of Smac into the cytosol. Activation of PKC delta rescues the interaction during paclitaxel exposure and suppresses the paclitaxel-mediated cell death. However, under these conditions the complex is mainly found in the cytosol suggesting that cytosolic Smac can be bound by PKC delta when PKC is activated. The data unravel a previously unrecognized interaction and suggest that PKC delta by associating with Smac may prevent its apoptotic effects. Structured summary of protein interactions: PKC delta physically interacts with SMAC by anti bait coimmunoprecipitation (View Interaction: 1, 2, 3, 4) XIAP physically interacts with SMAC by anti tag coimmunoprecipitation (View interaction) (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
