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[摘要]：In this paper we report the development of chromatographic methods for the separation of 8 biologically active hydroxycholesterols (OHC's) which include the single-hydroxyl addition species 7 alpha-OHC, 7 beta-OHC, 25-OHC and 27-OHC, together with the double-hydroxyl addition species 7 alpha, 25-OHC, 7 beta, 25-OHC, 7 alpha, 27-OHC, and 7 beta, 27-OHC. Four complementary techniques were employed (gas chromatography, normal phase and reversed phase high performance liquid chromatography, and supercritical fluid chromatography), and for each of the techniques, an optimized method for the separation of all eight compounds in a mixture is presented. |
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