[摘要]：BACKGROUND & AIMS: Lysozyme has an important rote in preventing bacterial infection. In the gastrointestinal tract, lysozyme IS thought to be mainly expressed by Paneth cells OF the crypt epithelium. We Investigated its expression in the Peyer's patch, a major intestinal sire of antigen Sampling and pathogen entry. METHODS: We performed immunostaining on normal and Salmonella Typhimurium-infected intestinal samples and analyzed them by confocal microscopy and flow cytometry. RESULTS: In Peyer's patch of mouse, rat, and human, lysozyme was strongly expressed in the germinal center OF Follicles by tingible body macrophages and in the subepithelial dome by a subset of myeloid dendritic cells (DC). Among DC subsets from mouse Peyer's patches, these lysozyme-expressing DC displayed the highest surface expression of class 11 major histocompatibility complex and costimulatory molecules the), were the most efficient at capturing microspheres in vitro. Moreover, they were the main DC subset involved in bacterial pathogen Uptake and in dead cell clearance, including M cells. CONCLUSIONS: The sub-epithelial. dome of Peyer's patches contains a unique population of intestinal DC that secretes high levels of lysozyme and internalizes bacteria and dead cells.
