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[摘要]：Early growth response factor 1 (Egr-1) is induced during activity deprivation-dependent apoptosis in cerebellar granule neurons and plays a critical role in neuronal apoptosis. It has been proposed that c-Jun lies downstream of the Egr-dependent proapoptotic signaling cascade in cerebellar granule neurons. However, our data show that neither suppressing Egr activity using a dominant-negative mutant nor specifically silencing Egr-1 using small interfering RNA inhibited the activation and expression of c-Jun after activity deprivation in cerebellar granule neurons. Furthermore, two inhibitors, mithramycin A and chromomycin A3, which repressed transcription by selectively displacing GC-rich DNA-binding transcription factors (including those of the Egr family), did not attenuate the activation and expression of c-Jun during neuronal apoptosis. We conclude that c-Jun induction is independent of Egr during apoptosis induced by activity deprivation in cerebellar granule neurons. NeuroReport 23:67-72 (C) 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins. |
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