[摘要]：Recently, the infarct reducing and cardioprotective effects of phosphodiesterase-5-inhibitors were described. In this Study, we investigated these effects on ischemia/reperfusion injury in a rat model of heart transplantation. Three groups were assigned for our study: a vardenafil preconditioning group, an ischemic control, and a nonischemic control. Hemodynamic parameters were significantly increased in the vardenafil group (P-max: 82 +/- 4 vs. 110 +/- 12 vs. 127 +/- 13 mm Hg; dP/dt(max): 1740 +/- 116 vs. 3197 +/- 599 vs. 4397 +/- 602 mm Hg/sec; ischemic control vs. vardenafil vs. nonischemic control; P<0.05 vs. ischemic control). Furthermore, we recorded increased ATP levels and significantly less apoptosis in the treatment group after terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (apoptosis index: 27.23%+/- 1.54% vs. 16.77%+/- 1.42% vs. 18.86%+/- 1.07%; ischemic control vs. vardenafil vs. nonischemic control; P<0.05 vs. ischemic control). Our Current results support the concept that the cGMP-PKG-pathway plays an important role in ischemia/reperfusion injury. We could show that up-regulating this pathway has a preconditioning-like effect and can effectively reduce ischemia/reperfusion injury.
