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[摘要]：Batch production of biohydrogen from cassava wastewater pretreated with (i) sonication, (ii) OPTIMASH BG (R) (enzyme), and (iii) alpha-amylase (enzyme) were investigated using anaerobic seed sludge subjected to heat pretreatment at 105 degrees C for 90 min. Hydrogen yield at pH 7.0 for cassava wastewater pretreated with sonication for 45 min using anaerobic seed sludge was 0.913 mol H(2)/g COD. Results from pretreatment with OPTIMASH BG (R) at 0.20% and pH 7 showed a hydrogen yield of 4.24 mol H(2)/g COD. Superior results were obtained when the wastewater was pretreated with alpha-amylase at 0.20% at pH 7 with a hydrogen yield of 5.02 mol H(2)/g COD. In all cases, no methane production was observed when using heat-treated sludge as seed inoculum. Percentage COD removal was found to be highest (60%) using alpha-amylase as pretreatment followed by OPTIMASH BG (R) at 54% and sonication (40% reduction rate). Results further suggested that cassava wastewater is one of the potential sources of renewable biomass to produce hydrogen. (C) 2011 Elsevier Ltd. All rights reserved. |
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