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Lifetimes of photosystem I and II proteins in the cyanobacterium Synechocystis sp PCC 6803

  作者 Yao, DCI; Brune, DC; Vermaas, WFJ  
  选自 期刊  FEBS Letters;  卷期  2012年586-2;  页码  169-173  
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[摘要]The half-life times of photosystem I and II proteins were determined using N-15-labeling and mass spectrometry. The half-life times (30-75 h for photosystem I components and <1-11 h for the large photosystem II proteins) were similar when proteins were isolated from monomeric vs. oligomeric complexes on Blue-Native gels, suggesting that the two forms of both photosystems can interchange on a timescale of <1 h or that only one form of each photosystem exists in thylakoids in vivo. The half-life times of proteins associated with either photosystem generally were unaffected by the absence of Small Cab-like proteins. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

 
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